Hotspot

rpl32-tRNA-Pro

Genome · 66285-66715 · 431 bp

Back to Coleochaete

Pi 0.0831

MarkerSeek score 80.20

Primer available yes

Label rpl32-tRNA-Pro

Feature Metadata

label_name: rpl32-tRNA-Pro
feature_type: igs
region: Genome
length_bp: 431
variable_sites: 35
pi: 0.0831
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-tRNA-Pro_p1	AGATTTAGCCAAGGATAGTGCT	CGCAGTTTGGTAGCGTATCA	40.9	50.0	56.9	58.4	596-606 bp	1.0	58.6
rpl32-tRNA-Pro_p2	AGATTTAGCCAAGGATAGTGCT	GGTAGCGTATCATCTTGGGG	40.9	55.0	56.9	57.5	588-598 bp	1.0	58.0
rpl32-tRNA-Pro_p3	AGATTTAGCCAAGGATAGTGCT	AGCGTATCATCTTGGGGTGA	40.9	50.0	56.9	58.5	585-595 bp	1.0	57.6
rpl32-tRNA-Pro_p4	AGATTTAGCCAAGGATAGTGCT	GAGGTCGTGGGTTCAAATCC	40.9	55.0	56.9	58.5	563-573 bp	1.0	57.5
rpl32-tRNA-Pro_p5	AGATTTAGCCAAGGATAGTGCT	GGAGGTCGTGGGTTCAAATC	40.9	55.0	56.9	58.5	564-574 bp	1.0	57.5