Hotspot

rps3

LSC · 91728-92384 · 657 bp

Back to Cojoba

Pi 0.0167
MarkerSeek score 57.20
Primer available yes
Label rps3

Feature Metadata

label_name
rps3
feature_type
gene
region
LSC
length_bp
657
variable_sites
11
pi
0.0167
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps3_p1 ACGTTAACAATAAGGCAAAGT TCAAAGACGCAAATACGCCA 33.3 45.0 54.0 58.5 791 bp 1.0 41.1
rps3_p2 ACGTTAACAATAAGGCAAAGT CAAAGACGCAAATACGCCAT 33.3 45.0 54.0 57.5 790 bp 1.0 41.1
rps3_p3 AACGTTAACAATAAGGCAAAGT TCAAAGACGCAAATACGCCA 31.8 45.0 54.9 58.5 792 bp 1.0 41.1
rps3_p4 ACGTTAACAATAAGGCAAAGTT TCAAAGACGCAAATACGCCA 31.8 45.0 54.9 58.5 791 bp 1.0 41.1
rps3_p5 ACGTTAACAATAAGGCAAAGTT CAAAGACGCAAATACGCCAT 31.8 45.0 54.9 57.5 790 bp 1.0 41.1