Hotspot

rpl2-rpl23

IRb · 94966-94983 · 18 bp

Back to Cojoba

Pi 0.0556
MarkerSeek score 81.90
Primer available yes
Label rpl2-rpl23

Feature Metadata

label_name
rpl2-rpl23
feature_type
igs
region
IRb
length_bp
18
variable_sites
1
pi
0.0556
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl2-rpl23_p1 CACTTGACTGTCTACGGCTC GGTAGAAGGATGGGGCCTAT 55.0 55.0 58.0 58.0 192 bp 1.0 79.9
rpl2-rpl23_p2 CCACAACGATGCTGTCCATA GGTAGAAGGATGGGGCCTAT 50.0 55.0 58.0 58.0 239 bp 1.0 79.8
rpl2-rpl23_p3 GACTGTCTACGGCTCCATTG GGTAGAAGGATGGGGCCTAT 55.0 55.0 58.1 58.0 187 bp 1.0 79.5
rpl2-rpl23_p4 CACTTGACTGTCTACGGCTC CCCGGTAAAGGGTAGAAGGA 55.0 55.0 58.0 58.1 202 bp 1.0 79.2
rpl2-rpl23_p5 CCACAACGATGCTGTCCATA CCCGGTAAAGGGTAGAAGGA 50.0 55.0 58.0 58.1 249 bp 1.0 79.1