Hotspot

psbE-petL

LSC · 72494-73795 · 1302 bp

Back to Cojoba

Pi 0.00694
MarkerSeek score 63.10
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1302
variable_sites
9
pi
0.00694
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 TGACAAATAACCAACCCGCA GTCGTATCTTGCTCAGACCA 45.0 50.0 58.0 57.1 1500-1505 bp 1.0 73.1
psbE-petL_p2 CAACCCGCAATGAATAGGGA GTCGTATCTTGCTCAGACCA 50.0 50.0 57.9 57.1 1489-1494 bp 1.0 72.8
psbE-petL_p3 AGAACGTTCTCCTGTGCTTC GTCGTATCTTGCTCAGACCA 50.0 50.0 57.8 57.1 1412-1417 bp 1.0 72.0
psbE-petL_p4 GTTCTCCTGTGCTTCCAGAC GTCGTATCTTGCTCAGACCA 55.0 50.0 58.2 57.1 1407-1412 bp 1.0 71.7
psbE-petL_p5 ACCAACCCGCAATGAATAGG GTCGTATCTTGCTCAGACCA 50.0 50.0 58.2 57.1 1491-1496 bp 1.0 71.3