Hotspot

cemA-petA

LSC · 69063-69286 · 224 bp

Back to Cojoba

Pi 0.0179
MarkerSeek score 62.80
Primer available yes
Label cemA-petA

Feature Metadata

label_name
cemA-petA
feature_type
igs
region
LSC
length_bp
224
variable_sites
4
pi
0.0179
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
cemA-petA_p1 CGTGTATCTCCGTCACTTGT TTGAAATGGGTGCTCGAGTT 50.0 45.0 57.4 57.7 372 bp 1.0 73.6
cemA-petA_p2 CGTGTATCTCCGTCACTTGT TTTGAAATGGGTGCTCGAGT 50.0 45.0 57.4 57.7 373 bp 1.0 73.6
cemA-petA_p3 CGTGTATCTCCGTCACTTGT GCATTTGAAATGGGTGCTCG 50.0 50.0 57.4 58.4 376 bp 1.0 72.9
cemA-petA_p4 TCCGGTCTTGTTTCCACTTT TTGAAATGGGTGCTCGAGTT 45.0 45.0 57.3 57.7 444 bp 1.0 72.8
cemA-petA_p5 TCCGGTCTTGTTTCCACTTT TTTGAAATGGGTGCTCGAGT 45.0 45.0 57.3 57.7 445 bp 1.0 72.8