Hotspot

trnM-CAU-atpE

LSC · 59440-59721 · 282 bp

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Pi 0.0182
MarkerSeek score 65.30
Primer available yes
Label trnM-CAU-atpE

Feature Metadata

label_name
trnM-CAU-atpE
feature_type
igs
region
LSC
length_bp
282
variable_sites
5
pi
0.0182
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-atpE_p1 TATTGCTTTCATACGGCGGG CTCTCAGACGAGCTAGGACA 50.0 55.0 58.4 58.0 374-381 bp 1.0 79.0
trnM-CAU-atpE_p2 TATTGCTTTCATACGGCGGG CGAGCTAGGACACGAGTAGA 50.0 55.0 58.4 58.1 366-373 bp 1.0 78.8
trnM-CAU-atpE_p3 TATTGCTTTCATACGGCGGG TCTCAGACGAGCTAGGACAC 50.0 55.0 58.4 58.3 373-380 bp 1.0 77.2
trnM-CAU-atpE_p4 GTATTGCTTTCATACGGCGG CTCTCAGACGAGCTAGGACA 50.0 55.0 57.3 58.0 375-382 bp 1.0 77.0
trnM-CAU-atpE_p5 GTATTGCTTTCATACGGCGG CGAGCTAGGACACGAGTAGA 50.0 55.0 57.3 58.1 367-374 bp 1.0 76.8