Hotspot

trnT-UGU-trnL-UAA

LSC · 52359-53914 · 1556 bp

Back to Cojoba

Pi 0.00582
MarkerSeek score 62.60
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
1556
variable_sites
9
pi
0.00582
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GGAATCGAACCGATGACCAT ACCAATTTCGCCATATCCCC 50.0 50.0 57.8 58.0 1638-1714 bp 1.0 78.9
trnT-UGU-trnL-UAA_p2 GGAATCGAACCGATGACCAT TAGCGTCTACCAATTTCGCC 50.0 50.0 57.8 58.1 1646-1722 bp 1.0 78.4
trnT-UGU-trnL-UAA_p3 AAATGCGACGCTCTAACCTC ACCAATTTCGCCATATCCCC 50.0 50.0 58.4 58.0 1610-1686 bp 1.0 78.0
trnT-UGU-trnL-UAA_p4 AAATGCGACGCTCTAACCTC TAGCGTCTACCAATTTCGCC 50.0 50.0 58.4 58.1 1618-1694 bp 1.0 77.5
trnT-UGU-trnL-UAA_p5 GGAATCGAACCGATGACCAT AGTCCGTAGCGTCTACCAAT 50.0 50.0 57.8 58.2 1652-1728 bp 1.0 77.1