Hotspot

trnG-GCC-trnfM-CAU

LSC · 42470-42736 · 267 bp

Back to Cojoba

Pi 0.0466
MarkerSeek score 67.20
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
267
variable_sites
9
pi
0.0466
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.72
markerseek_score
67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 CTTTGCCAAAGAGAAGACGC CTTGAGGTCACGGGTTCAAA 50.0 50.0 57.7 58.0 274-344 bp 1.0 83.4
trnG-GCC-trnfM-CAU_p2 CTTTGCCAAAGAGAAGACGC AGCTCGCAAGGCTCATAATC 50.0 50.0 57.7 58.1 293-363 bp 1.0 82.6
trnG-GCC-trnfM-CAU_p3 CTTTGCCAAAGAGAAGACGC GCTCGCAAGGCTCATAATCT 50.0 50.0 57.7 58.1 292-362 bp 1.0 82.6
trnG-GCC-trnfM-CAU_p4 CTTTGCCAAAGAGAAGACGC TGAGGTCACGGGTTCAAATC 50.0 50.0 57.7 57.8 272-342 bp 1.0 82.3
trnG-GCC-trnfM-CAU_p5 CTTTGCCAAAGAGAAGACGC GTAGCTCGCAAGGCTCATAA 50.0 50.0 57.7 57.8 295-365 bp 1.0 81.8