Hotspot

psbZ-trnG-GCC

LSC · 41628-42398 · 771 bp

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Pi 0.00596
MarkerSeek score 64.70
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
771
variable_sites
4
pi
0.00596
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.87
markerseek_score
64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 CTTCTCCTGATGGTTGGTCG GCGTCTTCTCTTTGGCAAAG 55.0 50.0 58.0 57.7 836-926 bp 1.0 81.7
psbZ-trnG-GCC_p2 CTTCTCCTGATGGTTGGTCG ATACATAAATAGGGGGCGCG 55.0 50.0 58.0 57.6 880-970 bp 1.0 81.0
psbZ-trnG-GCC_p3 TTGCTTCTCCTGATGGTTGG GCGTCTTCTCTTTGGCAAAG 50.0 50.0 57.8 57.7 839-929 bp 1.0 80.1
psbZ-trnG-GCC_p4 TCCTGATGGTTGGTCGAGTA GCGTCTTCTCTTTGGCAAAG 50.0 50.0 57.8 57.7 832-922 bp 1.0 80.0
psbZ-trnG-GCC_p5 GCTTCTCCTGATGGTTGGTC GCGTCTTCTCTTTGGCAAAG 55.0 50.0 58.3 57.7 837-927 bp 1.0 79.7