Hotspot

trnR-UCU-atpA

LSC · 12118-12642 · 525 bp

Back to Cojoba

Pi 0.0114
MarkerSeek score 63.80
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
525
variable_sites
6
pi
0.0114
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 GGTTCAAATCCTATTGGACGC CATTCACTCGGGAAGCAGAA 47.6 50.0 57.3 57.9 639-661 bp 1.0 66.4
trnR-UCU-atpA_p2 GGTTCAAATCCTATTGGACGC AAGACATTCACTCGGGAAGC 47.6 50.0 57.3 57.9 643-665 bp 1.0 66.4
trnR-UCU-atpA_p3 GGTTCAAATCCTATTGGACGC GGAAGCAGAAGCCCTTTTGA 47.6 50.0 57.3 58.4 629-651 bp 1.0 64.2
trnR-UCU-atpA_p4 GGTTCAAATCCTATTGGACGC ATTCACTCGGGAAGCAGAAG 47.6 50.0 57.3 57.6 638-660 bp 1.0 64.1
trnR-UCU-atpA_p5 TGGATAGGACAGAGGTCTTCT CATTCACTCGGGAAGCAGAA 47.6 50.0 56.9 57.9 674-696 bp 1.0 63.5