Hotspot

trnS-GCU-trnG-UCC

LSC · 9520-10898 · 1379 bp

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Pi 0.0131
MarkerSeek score 64.60
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
1379
variable_sites
18
pi
0.0131
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA TGAATCAAACGAGGGATCCC 50.0 50.0 58.0 57.3 1491-1524 bp 1.0 75.6
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC TGAATCAAACGAGGGATCCC 50.0 50.0 58.0 57.3 1490-1523 bp 1.0 75.5
trnS-GCU-trnG-UCC_p3 ACGGAAAGAGAGGGATTCGA TGAATCAAACGAGGGATCCC 50.0 50.0 58.2 57.3 1545-1578 bp 1.0 74.3
trnS-GCU-trnG-UCC_p4 CAATCCGACGCTTTAGTCCA GGATCCATAGAACGAATCGCA 50.0 47.6 58.0 58.0 1459-1492 bp 1.0 73.1
trnS-GCU-trnG-UCC_p5 AATCCGACGCTTTAGTCCAC GGATCCATAGAACGAATCGCA 50.0 47.6 58.0 58.0 1458-1491 bp 1.0 73.0