Hotspot

trnK-UUU-rps16

LSC · 4252-5749 · 1498 bp

Back to Cojoba

Pi 0.043
MarkerSeek score 83.70
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1498
variable_sites
61
pi
0.043
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGATGGTATGGACGAATCC AAAAAGGCGCTCAACCTACA 55.0 45.0 57.9 58.0 1699-1722 bp 1.0 85.7
trnK-UUU-rps16_p2 CCGATGGTATGGACGAATCC CCTTGAAAAAGGCGCTCAAC 55.0 50.0 57.9 58.2 1704-1727 bp 1.0 84.0
trnK-UUU-rps16_p3 CCGATGGTATGGACGAATCC CTTGAAAAAGGCGCTCAACC 55.0 50.0 57.9 58.2 1703-1726 bp 1.0 84.0
trnK-UUU-rps16_p4 CCGATGGTATGGACGAATCC TGAAAAAGGCGCTCAACCTA 55.0 45.0 57.9 57.7 1701-1724 bp 1.0 83.7
trnK-UUU-rps16_p5 CCGATGGTATGGACGAATCC AGGCGGGTGTTTTTATGGAA 55.0 45.0 57.9 57.7 1654-1677 bp 1.0 83.7