Hotspot

trnG-GCC

LSC · 13165-13240 · 76 bp

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Pi 0.0435
MarkerSeek score 55.80
Primer available yes
Label trnG-GCC

Feature Metadata

label_name
trnG-GCC
feature_type
tRNA
region
LSC
length_bp
76
variable_sites
6
pi
0.0435
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC_p1 GGTCGACCCAGGTGGATATA TTTGATTCGGGTGCAAAGGA 55.0 45.0 58.0 58.0 416-423 bp 1.0 86.4
trnG-GCC_p2 TCGACCCAGGTGGATATACC TTTGATTCGGGTGCAAAGGA 55.0 45.0 58.0 58.0 414-421 bp 1.0 86.4
trnG-GCC_p3 AAAATGAAACGGTCGACCCA TTTGATTCGGGTGCAAAGGA 45.0 45.0 58.0 58.0 445-452 bp 1.0 85.8
trnG-GCC_p4 GGTCGACCCAGGTGGATATA AGCCTTCCAAGCTAACGATG 55.0 50.0 58.0 58.0 856-864 bp 1.0 84.0
trnG-GCC_p5 TCGACCCAGGTGGATATACC AGCCTTCCAAGCTAACGATG 55.0 50.0 58.0 58.0 854-862 bp 1.0 84.0