Hotspot

rps2

LSC · 32211-32921 · 711 bp

Back to Coix

Pi 0.000703
MarkerSeek score 45.20
Primer available yes
Label rps2

Feature Metadata

label_name
rps2
feature_type
gene
region
LSC
length_bp
711
variable_sites
1
pi
0.000703
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps2_p1 TCCGTAATGGTAAGACGAAA TCTACGCAGTTGCTCAAGAA 40.0 45.0 54.1 57.2 799 bp 1.0 42.8
rps2_p2 TTCCGTAATGGTAAGACGAA TCTACGCAGTTGCTCAAGAA 40.0 45.0 54.1 57.2 800 bp 1.0 42.8
rps2_p3 TCCGTAATGGTAAGACGAAAA TCTACGCAGTTGCTCAAGAA 38.1 45.0 55.0 57.2 799 bp 1.0 41.6
rps2_p4 TTCCGTAATGGTAAGACGAAA TCTACGCAGTTGCTCAAGAA 38.1 45.0 55.0 57.2 800 bp 1.0 41.6
rps2_p5 TCAAGCTATTTTGGCTCTTTCT TCTACGCAGTTGCTCAAGAA 36.4 45.0 56.0 57.2 843 bp 1.0 41.4