Hotspot

rpl16

LSC · 79914-81409 · 1496 bp

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Pi 0.000668
MarkerSeek score 45.20
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1496
variable_sites
2
pi
0.000668
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 TCCAGAAAGAAAAACCAGGGG AGACAAGAACCTTGTTTTTCCA 47.6 36.4 58.0 56.3 1652-1653 bp 1.0 42.2
rpl16_p2 TCCAGAAAGAAAAACCAGGGG ACCTTGTTTTTCCAAAAATCCCT 47.6 34.8 58.0 57.3 1644-1645 bp 1.0 42.1
rpl16_p3 CCAGAAAGAAAAACCAGGGGT AGACAAGAACCTTGTTTTTCCA 47.6 36.4 58.3 56.3 1651-1652 bp 1.0 40.1
rpl16_p4 CCAGAAAGAAAAACCAGGGGT ACCTTGTTTTTCCAAAAATCCCT 47.6 34.8 58.3 57.3 1643-1644 bp 1.0 40.1
rpl16_p5 TCCAGAAAGAAAAACCAGGGG AGACAAGAACCTTGTTTTTCCAA 47.6 34.8 58.0 57.0 1652-1653 bp 1.0 40.1