Hotspot

trnT-GGU-trnE-UUC

LSC · 16494-16958 · 465 bp

Back to Coix

Pi 0.0151
MarkerSeek score 52.60
Primer available yes
Label trnT-GGU-trnE-UUC

Feature Metadata

label_name
trnT-GGU-trnE-UUC
feature_type
igs
region
LSC
length_bp
465
variable_sites
14
pi
0.0151
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-trnE-UUC_p1 GACAGAAGTCATCGGTTCGA GCTGCCTCCTTGAAAGAGAG 50.0 55.0 57.4 58.3 543-550 bp 1.0 75.0
trnT-GGU-trnE-UUC_p2 GACAGAAGTCATCGGTTCGA TGTCCTGAACCACTAGACGA 50.0 50.0 57.4 57.7 522-529 bp 1.0 75.0
trnT-GGU-trnE-UUC_p3 GACAGAAGTCATCGGTTCGA CTGAACCACTAGACGATGGG 50.0 55.0 57.4 57.4 518-525 bp 1.0 72.6
trnT-GGU-trnE-UUC_p4 GACAGAAGTCATCGGTTCGA CCTGAACCACTAGACGATGG 50.0 55.0 57.4 57.4 519-526 bp 1.0 72.5
trnT-GGU-trnE-UUC_p5 GACAGAAGTCATCGGTTCGAA GCTGCCTCCTTGAAAGAGAG 47.6 55.0 58.1 58.3 543-550 bp 1.0 71.6