Hotspot

trnfM-CAU-trnT-GGU

LSC · 13600-16429 · 2830 bp

Back to Coix

Pi 0.00283
MarkerSeek score 39.00
Primer available yes
Label trnfM-CAU-trnT-GGU

Feature Metadata

label_name
trnfM-CAU-trnT-GGU
feature_type
igs
region
LSC
length_bp
2830
variable_sites
16
pi
0.00283
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
39.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnfM-CAU-trnT-GGU_p1 CCTCAAGGTTATGAGCCTCG TCGAACCGATGACTTCTGTC 55.0 50.0 57.8 57.4 2931-2932 bp 1.0 73.5
trnfM-CAU-trnT-GGU_p2 CCTCAAGGTTATGAGCCTCG CGAACCGATGACTTCTGTCT 55.0 50.0 57.8 57.4 2930-2931 bp 1.0 73.5
trnfM-CAU-trnT-GGU_p3 CCTCGTGAGCTACCAAACTG TCGAACCGATGACTTCTGTC 55.0 50.0 58.3 57.4 2916-2917 bp 1.0 73.1
trnfM-CAU-trnT-GGU_p4 CCTCGTGAGCTACCAAACTG CGAACCGATGACTTCTGTCT 55.0 50.0 58.3 57.4 2915-2916 bp 1.0 73.0
trnfM-CAU-trnT-GGU_p5 CCTCAAGGTTATGAGCCTCG CGAACCGATGACTTCTGTCTT 55.0 47.6 57.8 58.1 2930-2931 bp 1.0 70.2