Hotspot

trnG-GCC-trnfM-CAU

LSC · 13241-13525 · 285 bp

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Pi 0.037
MarkerSeek score 50.30
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
285
variable_sites
21
pi
0.037
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 GGTCGACCCAGGTGGATATA AGCCTTCCAAGCTAACGATG 55.0 50.0 58.0 58.0 856-864 bp 1.0 84.0
trnG-GCC-trnfM-CAU_p2 TCGACCCAGGTGGATATACC AGCCTTCCAAGCTAACGATG 55.0 50.0 58.0 58.0 854-862 bp 1.0 84.0
trnG-GCC-trnfM-CAU_p3 AAAATGAAACGGTCGACCCA AGCCTTCCAAGCTAACGATG 45.0 50.0 58.0 58.0 885-893 bp 1.0 83.7
trnG-GCC-trnfM-CAU_p4 GGTCGACCCAGGTGGATATA TTGTTTCAGCTCGGTGGAAA 55.0 45.0 58.0 58.0 1139-1147 bp 1.0 82.8
trnG-GCC-trnfM-CAU_p5 TCGACCCAGGTGGATATACC TTGTTTCAGCTCGGTGGAAA 55.0 45.0 58.0 58.0 1137-1145 bp 1.0 82.8