Hotspot

trnG-UCC-trnG-GCC

LSC · 13010-13164 · 155 bp

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Pi 0.0355
MarkerSeek score 50.80
Primer available yes
Label trnG-UCC-trnG-GCC

Feature Metadata

label_name
trnG-UCC-trnG-GCC
feature_type
igs
region
LSC
length_bp
155
variable_sites
11
pi
0.0355
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnG-GCC_p1 AATATAGGCCGTAGCAAGCG AGTAGTGTAGTTTTAAGTAGTCA 50.0 30.4 57.9 52.3 291 bp 0.25 10.0
trnG-UCC-trnG-GCC_p2 AATATAGGCCGTAGCAAGCG AAGTAGTGTAGTTTTAAGTAGTCA 50.0 29.2 57.9 53.1 292 bp 0.25 10.0
trnG-UCC-trnG-GCC_p3 CCGTAGCAAGCGTAGTTCAA AGTAGTGTAGTTTTAAGTAGTCA 50.0 30.4 58.3 52.3 283 bp 0.25 10.0
trnG-UCC-trnG-GCC_p4 TATAGGCCGTAGCAAGCGTA AGTAGTGTAGTTTTAAGTAGTCA 50.0 30.4 58.4 52.3 289 bp 0.25 10.0
trnG-UCC-trnG-GCC_p5 AGGAGAAGATACGGGTTCGA AGTAGTGTAGTTTTAAGTAGTCA 50.0 30.4 57.6 52.3 223 bp 0.25 10.0