primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5585	7120	1607-1610	1.000000	78.7
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	5585	7120	1618-1621	1.000000	78.2
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	20	20	45.0	55.0	58.1	57.8	8.1	16.9	0.350677	5585	7120	1611-1614	1.000000	77.6
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	TCATGTCCTTCAAGTCGCAC	CTCGGAGGTTCGAATCCTTC	20	20	50.0	55.0	58.2	57.8	0.0	16.9	0.415585	5585	7120	1622-1625	1.000000	77.1
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	TACTCGGAGGTTCGAATCCT	20	20	45.0	50.0	58.1	57.6	8.1	16.1	0.571278	5585	7120	1613-1616	1.000000	75.9
trnG-UCC-trnG-GCC_p1	trnG-UCC-trnG-GCC	1	AATATAGGCCGTAGCAAGCG	AGTAGTGTAGTTTTAAGTAGTCA	20	23	50.0	30.4	57.9	52.3	0.0	0.0	8.792871	13010	13164	291-291	0.250000	10.0
trnG-UCC-trnG-GCC_p2	trnG-UCC-trnG-GCC	2	AATATAGGCCGTAGCAAGCG	AAGTAGTGTAGTTTTAAGTAGTCA	20	24	50.0	29.2	57.9	53.1	0.0	0.0	8.953555	13010	13164	292-292	0.250000	10.0
trnG-UCC-trnG-GCC_p3	trnG-UCC-trnG-GCC	3	CCGTAGCAAGCGTAGTTCAA	AGTAGTGTAGTTTTAAGTAGTCA	20	23	50.0	30.4	58.3	52.3	0.0	0.0	9.007328	13010	13164	283-283	0.250000	10.0
trnG-UCC-trnG-GCC_p4	trnG-UCC-trnG-GCC	4	TATAGGCCGTAGCAAGCGTA	AGTAGTGTAGTTTTAAGTAGTCA	20	23	50.0	30.4	58.4	52.3	0.0	0.0	9.104530	13010	13164	289-289	0.250000	10.0
trnG-UCC-trnG-GCC_p5	trnG-UCC-trnG-GCC	5	AGGAGAAGATACGGGTTCGA	AGTAGTGTAGTTTTAAGTAGTCA	20	23	50.0	30.4	57.6	52.3	0.0	0.0	9.144538	13010	13164	223-223	0.250000	10.0
trnG-GCC_p1	trnG-GCC	1	GGTCGACCCAGGTGGATATA	TTTGATTCGGGTGCAAAGGA	20	20	55.0	45.0	58.0	58.0	24.8	0.0	0.017077	13165	13240	416-423	1.000000	86.4
trnG-GCC_p2	trnG-GCC	2	TCGACCCAGGTGGATATACC	TTTGATTCGGGTGCAAAGGA	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.017077	13165	13240	414-421	1.000000	86.4
trnG-GCC_p3	trnG-GCC	3	AAAATGAAACGGTCGACCCA	TTTGATTCGGGTGCAAAGGA	20	20	45.0	45.0	58.0	58.0	28.5	0.0	0.041615	13165	13240	445-452	1.000000	85.8
trnG-GCC_p4	trnG-GCC	4	GGTCGACCCAGGTGGATATA	AGCCTTCCAAGCTAACGATG	20	20	55.0	50.0	58.0	58.0	24.8	0.0	0.035208	13165	13240	856-864	1.000000	84.0
trnG-GCC_p5	trnG-GCC	5	TCGACCCAGGTGGATATACC	AGCCTTCCAAGCTAACGATG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.035208	13165	13240	854-862	1.000000	84.0
trnG-GCC-trnfM-CAU_p1	trnG-GCC-trnfM-CAU	1	GGTCGACCCAGGTGGATATA	AGCCTTCCAAGCTAACGATG	20	20	55.0	50.0	58.0	58.0	24.8	0.0	0.035208	13241	13525	856-864	1.000000	84.0
trnG-GCC-trnfM-CAU_p2	trnG-GCC-trnfM-CAU	2	TCGACCCAGGTGGATATACC	AGCCTTCCAAGCTAACGATG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.035208	13241	13525	854-862	1.000000	84.0
trnG-GCC-trnfM-CAU_p3	trnG-GCC-trnfM-CAU	3	AAAATGAAACGGTCGACCCA	AGCCTTCCAAGCTAACGATG	20	20	45.0	50.0	58.0	58.0	28.5	0.0	0.059746	13241	13525	885-893	1.000000	83.7
trnG-GCC-trnfM-CAU_p4	trnG-GCC-trnfM-CAU	4	GGTCGACCCAGGTGGATATA	TTGTTTCAGCTCGGTGGAAA	20	20	55.0	45.0	58.0	58.0	24.8	0.0	0.050932	13241	13525	1139-1147	1.000000	82.8
trnG-GCC-trnfM-CAU_p5	trnG-GCC-trnfM-CAU	5	TCGACCCAGGTGGATATACC	TTGTTTCAGCTCGGTGGAAA	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.050932	13241	13525	1137-1145	1.000000	82.8
trnfM-CAU-trnT-GGU_p1	trnfM-CAU-trnT-GGU	1	CCTCAAGGTTATGAGCCTCG	TCGAACCGATGACTTCTGTC	20	20	55.0	50.0	57.8	57.4	0.0	0.0	0.862633	13600	16429	2931-2932	1.000000	73.5
trnfM-CAU-trnT-GGU_p2	trnfM-CAU-trnT-GGU	2	CCTCAAGGTTATGAGCCTCG	CGAACCGATGACTTCTGTCT	20	20	55.0	50.0	57.8	57.4	0.0	0.0	0.869771	13600	16429	2930-2931	1.000000	73.5
trnfM-CAU-trnT-GGU_p3	trnfM-CAU-trnT-GGU	3	CCTCGTGAGCTACCAAACTG	TCGAACCGATGACTTCTGTC	20	20	55.0	50.0	58.3	57.4	0.0	0.0	0.916624	13600	16429	2916-2917	1.000000	73.1
trnfM-CAU-trnT-GGU_p4	trnfM-CAU-trnT-GGU	4	CCTCGTGAGCTACCAAACTG	CGAACCGATGACTTCTGTCT	20	20	55.0	50.0	58.3	57.4	0.0	0.0	0.923762	13600	16429	2915-2916	1.000000	73.0
trnfM-CAU-trnT-GGU_p5	trnfM-CAU-trnT-GGU	5	CCTCAAGGTTATGAGCCTCG	CGAACCGATGACTTCTGTCTT	20	21	55.0	47.6	57.8	58.1	0.0	0.0	1.283362	13600	16429	2930-2931	1.000000	70.2
trnT-GGU-trnE-UUC_p1	trnT-GGU-trnE-UUC	1	GACAGAAGTCATCGGTTCGA	GCTGCCTCCTTGAAAGAGAG	20	20	50.0	55.0	57.4	58.3	0.0	0.0	0.894807	16494	16958	543-550	1.000000	75.0
trnT-GGU-trnE-UUC_p2	trnT-GGU-trnE-UUC	2	GACAGAAGTCATCGGTTCGA	TGTCCTGAACCACTAGACGA	20	20	50.0	50.0	57.4	57.7	0.0	0.0	0.912957	16494	16958	522-529	1.000000	75.0
trnT-GGU-trnE-UUC_p3	trnT-GGU-trnE-UUC	3	GACAGAAGTCATCGGTTCGA	CTGAACCACTAGACGATGGG	20	20	50.0	55.0	57.4	57.4	0.0	0.0	1.217676	16494	16958	518-525	1.000000	72.6
trnT-GGU-trnE-UUC_p4	trnT-GGU-trnE-UUC	4	GACAGAAGTCATCGGTTCGA	CCTGAACCACTAGACGATGG	20	20	50.0	55.0	57.4	57.4	0.0	0.0	1.217676	16494	16958	519-526	1.000000	72.5
trnT-GGU-trnE-UUC_p5	trnT-GGU-trnE-UUC	5	GACAGAAGTCATCGGTTCGAA	GCTGCCTCCTTGAAAGAGAG	21	20	47.6	55.0	58.1	58.3	0.0	0.0	1.320679	16494	16958	543-550	1.000000	71.6
psbM-petN_p1	psbM-petN	1	TGTTCATTCTAGTTCCTACTGCC	TAGTAAGTCTCGCTTGGGCT	23	20	43.5	50.0	57.9	58.2	0.0	0.0	3.237452	18756	19555	951-951	1.000000	54.8
psbM-petN_p2	psbM-petN	2	TGTTCATTCTAGTTCCTACTGCC	GTAGTATGGGGGAGGAGTGG	23	20	43.5	60.0	57.9	58.3	0.0	0.0	3.367824	18756	19555	895-895	1.000000	53.8
psbM-petN_p3	psbM-petN	3	TGTTCATTCTAGTTCCTACTGCC	TAGTATGGGGGAGGAGTGGA	23	20	43.5	55.0	57.9	58.4	0.0	0.0	3.458256	18756	19555	894-894	1.000000	53.0
psbM-petN_p4	psbM-petN	4	GTTCATTCTAGTTCCTACTGCCT	TAGTAAGTCTCGCTTGGGCT	23	20	43.5	50.0	57.7	58.2	0.0	0.0	3.497529	18756	19555	950-950	1.000000	52.7
psbM-petN_p5	psbM-petN	5	TGTTCATTCTAGTTCCTACTGCC	CGCTAGTAGTATGGGGGAGG	23	20	43.5	60.0	57.9	58.5	0.0	0.0	3.539143	18756	19555	900-900	1.000000	52.4
trnC-GCA-rpoB_p1	trnC-GCA-rpoB	1	AAAGGATTTGCAGTCCCCTG	CTTCTCAAGTTCTTCCGCCA	20	20	50.0	50.0	58.1	57.8	14.6	0.0	0.240964	20619	21827	1363-1363	1.000000	78.1
trnC-GCA-rpoB_p2	trnC-GCA-rpoB	2	AAAGGATTTGCAGTCCCCTG	TCTCAAGTTCTTCCGCCAAG	20	20	50.0	50.0	58.1	57.8	14.6	0.0	0.240964	20619	21827	1361-1361	1.000000	78.1
trnC-GCA-rpoB_p3	trnC-GCA-rpoB	3	AAAGGATTTGCAGTCCCCTG	ACTTCTCAAGTTCTTCCGCC	20	20	50.0	50.0	58.1	57.8	14.6	0.0	0.242473	20619	21827	1364-1364	1.000000	78.1
trnC-GCA-rpoB_p4	trnC-GCA-rpoB	4	AAAGGATTTGCAGTCCCCTG	TTCCGCCAAGCCTTGATTAA	20	20	50.0	45.0	58.1	57.8	14.6	5.9	0.279264	20619	21827	1351-1351	1.000000	77.8
trnC-GCA-rpoB_p5	trnC-GCA-rpoB	5	AAAGGATTTGCAGTCCCCTG	CTTCCGCCAAGCCTTGATTA	20	20	50.0	50.0	58.1	58.2	14.6	5.9	0.320939	20619	21827	1352-1352	1.000000	77.5
rps2_p1	rps2	1	TCCGTAATGGTAAGACGAAA	TCTACGCAGTTGCTCAAGAA	20	20	40.0	45.0	54.1	57.2	0.0	2.3	4.666442	32211	32921	799-799	1.000000	42.8
rps2_p2	rps2	2	TTCCGTAATGGTAAGACGAA	TCTACGCAGTTGCTCAAGAA	20	20	40.0	45.0	54.1	57.2	0.0	2.3	4.666442	32211	32921	800-800	1.000000	42.8
rps2_p3	rps2	3	TCCGTAATGGTAAGACGAAAA	TCTACGCAGTTGCTCAAGAA	21	20	38.1	45.0	55.0	57.2	0.0	2.3	4.811847	32211	32921	799-799	1.000000	41.6
rps2_p4	rps2	4	TTCCGTAATGGTAAGACGAAA	TCTACGCAGTTGCTCAAGAA	21	20	38.1	45.0	55.0	57.2	0.0	2.3	4.811847	32211	32921	800-800	1.000000	41.6
rps2_p5	rps2	5	TCAAGCTATTTTGGCTCTTTCT	TCTACGCAGTTGCTCAAGAA	22	20	36.4	45.0	56.0	57.2	0.0	2.3	4.832574	32211	32921	843-843	1.000000	41.4
psaA-ycf3_p1	psaA-ycf3	1	GGGGCCCTTAGCTATTGTTC	TGGTTTGATCAAGCTGCTGA	20	20	55.0	45.0	58.0	57.7	24.4	19.9	0.292474	43865	44474	841-841	1.000000	77.8
psaA-ycf3_p2	psaA-ycf3	2	GTCTGGCCCATTCCTCAAAA	TGGTTTGATCAAGCTGCTGA	20	20	50.0	45.0	58.1	57.7	0.0	19.9	0.351778	43865	44474	806-806	1.000000	77.3
psaA-ycf3_p3	psaA-ycf3	3	GGGGCCCTTAGCTATTGTTC	GCTATAGCGCTTACTCCAGG	20	20	55.0	55.0	58.0	57.6	24.4	18.7	0.373057	43865	44474	808-808	1.000000	77.1
psaA-ycf3_p4	psaA-ycf3	4	GTCTGGCCCATTCCTCAAAA	GCTATAGCGCTTACTCCAGG	20	20	50.0	55.0	58.1	57.6	0.0	18.7	0.432361	43865	44474	773-773	1.000000	76.6
psaA-ycf3_p5	psaA-ycf3	5	GGTTCCGACGGACGAATAAT	TGGTTTGATCAAGCTGCTGA	20	20	50.0	45.0	57.8	57.7	18.1	19.9	0.489521	43865	44474	744-744	1.000000	76.2
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	ATTTGAACCGATGACCCTCG	ACAATCAAGTCCGTAGCGTC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.032006	48420	49215	897-897	1.000000	79.9
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	CGATGACCCTCGCATTACAA	ACAATCAAGTCCGTAGCGTC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.073242	48420	49215	889-889	1.000000	79.6
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	ACAATCAAGTCCGTAGCGTC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.140570	48420	49215	867-867	1.000000	79.1
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	ATTTGAACCGATGACCCTCG	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	58.0	58.2	0.0	10.2	0.272624	48420	49215	887-887	1.000000	78.0
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	ATTTGAACCGATGACCCTCG	CAATCAAGTCCGTAGCGTCT	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.303758	48420	49215	896-896	1.000000	77.7
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	CTAACCGGGGCTAAAAGTCC	GGTTCGAATCCGTATAGCCC	20	20	55.0	55.0	58.0	57.9	6.3	0.0	0.110083	52442	53380	1065-1065	1.000000	79.2
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	CTAACCGGGGCTAAAAGTCC	AGCCCAATGCAGAAAAATGC	20	20	55.0	45.0	58.0	57.9	6.3	0.0	0.137641	52442	53380	1158-1158	1.000000	79.0
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	CTAACCGGGGCTAAAAGTCC	CGGTTCGAATCCGTATAGCC	20	20	55.0	55.0	58.0	58.3	6.3	22.8	0.339465	52442	53380	1066-1066	1.000000	77.4
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TAACCGGGGCTAAAAGTCCT	GGTTCGAATCCGTATAGCCC	20	20	50.0	55.0	58.3	57.9	6.3	0.0	0.415737	52442	53380	1064-1064	1.000000	76.7
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TAACCGGGGCTAAAAGTCCT	AGCCCAATGCAGAAAAATGC	20	20	50.0	45.0	58.3	57.9	6.3	0.0	0.443295	52442	53380	1157-1157	1.000000	76.5
rbcL-rpl23_p1	rbcL-rpl23	1	GGCTTCAGGGGGTATTCATG	GTCATTGCAATTGCCGGAAA	20	20	55.0	45.0	58.0	57.9	0.0	26.9	0.090530	58611	58899	1312-1312	1.000000	80.6
rbcL-rpl23_p2	rbcL-rpl23	2	TCTCATTATTGCCGCGACAA	GTCATTGCAATTGCCGGAAA	20	20	45.0	45.0	58.0	57.9	11.0	26.9	0.094551	58611	58899	1602-1602	1.000000	80.3
rbcL-rpl23_p3	rbcL-rpl23	3	TTGTCTCATTATTGCCGCGA	GTCATTGCAATTGCCGGAAA	20	20	45.0	45.0	58.0	57.9	8.2	26.9	0.094551	58611	58899	1605-1605	1.000000	80.3
rbcL-rpl23_p4	rbcL-rpl23	4	GGCTTCAGGGGGTATTCATG	AGAAGAGTTCGACCCAATGC	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.113722	58611	58899	714-714	1.000000	80.1
rbcL-rpl23_p5	rbcL-rpl23	5	GGCTTCAGGGGGTATTCATG	GAAGAGTTCGACCCAATGCT	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.113722	58611	58899	713-713	1.000000	80.1
psbE-petL_p1	psbE-petL	1	TGACAAATAACCAACCCGCA	AAGCAGCCAGTAGAAAACCG	20	20	45.0	50.0	58.0	58.5	0.0	0.0	0.493582	64497	65757	1419-1421	1.000000	76.1
psbE-petL_p2	psbE-petL	2	TGACAAATAACCAACCCGCA	GCAGCCAGTAGAAAACCGAA	20	20	45.0	50.0	58.0	58.5	0.0	0.0	0.497931	64497	65757	1417-1419	1.000000	76.1
psbE-petL_p3	psbE-petL	3	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCG	20	20	50.0	50.0	57.9	58.5	6.4	0.0	0.527577	64497	65757	1408-1410	1.000000	75.8
psbE-petL_p4	psbE-petL	4	CAACCCGCAATGAATAGGGA	GCAGCCAGTAGAAAACCGAA	20	20	50.0	50.0	57.9	58.5	6.4	0.0	0.531926	64497	65757	1406-1408	1.000000	75.8
psbE-petL_p5	psbE-petL	5	ACCAACCCGCAATGAATAGG	AAGCAGCCAGTAGAAAACCG	20	20	50.0	50.0	58.2	58.5	0.0	0.0	0.713535	64497	65757	1410-1412	1.000000	74.3
rpl16_p1	rpl16	1	TCCAGAAAGAAAAACCAGGGG	AGACAAGAACCTTGTTTTTCCA	21	22	47.6	36.4	58.0	56.3	0.0	12.8	4.734947	79914	81409	1652-1653	1.000000	42.2
rpl16_p2	rpl16	2	TCCAGAAAGAAAAACCAGGGG	ACCTTGTTTTTCCAAAAATCCCT	21	23	47.6	34.8	58.0	57.3	0.0	0.7	4.751262	79914	81409	1644-1645	1.000000	42.1
rpl16_p3	rpl16	3	CCAGAAAGAAAAACCAGGGGT	AGACAAGAACCTTGTTTTTCCA	21	22	47.6	36.4	58.3	56.3	0.0	12.8	5.014507	79914	81409	1651-1652	1.000000	40.1
rpl16_p4	rpl16	4	CCAGAAAGAAAAACCAGGGGT	ACCTTGTTTTTCCAAAAATCCCT	21	23	47.6	34.8	58.3	57.3	0.0	0.7	5.030822	79914	81409	1643-1644	1.000000	40.1
rpl16_p5	rpl16	5	TCCAGAAAGAAAAACCAGGGG	AGACAAGAACCTTGTTTTTCCAA	21	23	47.6	34.8	58.0	57.0	0.0	12.8	5.036274	79914	81409	1652-1653	1.000000	40.1
rpl22_p1	rpl22	1	ACCAAGTCTGAAACCGAGTG	GGTAGGCGGGTAATAACCTT	20	20	50.0	50.0	57.8	56.7	0.0	0.0	1.549597	82289	82735	569-569	1.000000	69.1
rpl22_p2	rpl22	2	CCAAGTCTGAAACCGAGTGG	GGTAGGCGGGTAATAACCTT	20	20	55.0	50.0	58.5	56.7	0.0	0.0	1.796177	82289	82735	568-568	1.000000	67.2
rpl22_p3	rpl22	3	CAAGTCTGAAACCGAGTGGA	GGTAGGCGGGTAATAACCTT	20	20	50.0	50.0	57.5	56.7	0.0	0.0	1.835407	82289	82735	567-567	1.000000	66.9
rpl22_p4	rpl22	4	CAAGTCTGAAACCGAGTGGAT	GGTAGGCGGGTAATAACCTT	21	20	47.6	50.0	58.0	56.7	0.0	0.0	2.354704	82289	82735	567-567	1.000000	62.7
rpl22_p5	rpl22	5	TGGTGATTTTGGGTTGTACCA	GGTAGGCGGGTAATAACCTT	21	20	42.9	50.0	57.7	56.7	0.0	0.0	2.623410	82289	82735	586-586	1.000000	60.5