Hotspot

rpl16

LSC · 81916-83322 · 1407 bp

Back to Coffea

Pi 0.00748
MarkerSeek score 57.00
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1407
variable_sites
30
pi
0.00748
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 TCAATGCAAAGGACGAAGAA AGAAAGCTAAACCTCTTCATTCT 40.0 34.8 55.6 55.6 1577-1601 bp 0.909091 39.7
rpl16_p2 TTCAATGCAAAGGACGAAGA AGAAAGCTAAACCTCTTCATTCT 40.0 34.8 55.6 55.6 1578-1602 bp 0.909091 39.7
rpl16_p3 TCAATGCAAAGGACGAAGAAA AGAAAGCTAAACCTCTTCATTCT 38.1 34.8 56.4 55.6 1577-1601 bp 0.909091 39.7
rpl16_p4 TTCAATGCAAAGGACGAAGAA AGAAAGCTAAACCTCTTCATTCT 38.1 34.8 56.4 55.6 1578-1602 bp 0.909091 39.7
rpl16_p5 TTTCAATGCAAAGGACGAAGA AGAAAGCTAAACCTCTTCATTCT 38.1 34.8 56.4 55.6 1579-1603 bp 0.909091 39.7