Hotspot

rps18-rpl20

LSC · 69080-69300 · 221 bp

Back to Coffea

Pi 0.02
MarkerSeek score 56.40
Primer available yes
Label rps18-rpl20

Feature Metadata

label_name
rps18-rpl20
feature_type
igs
region
LSC
length_bp
221
variable_sites
11
pi
0.02
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps18-rpl20_p1 TGAAAGAACCGAGTCGACTG TCGATTGTAAAGAATCCAGTGGA 50.0 39.1 57.6 57.6 163-308 bp 0.727273 45.6
rps18-rpl20_p2 AACCGAGTCGACTGCTAGAA TCGATTGTAAAGAATCCAGTGGA 50.0 39.1 58.5 57.6 157-302 bp 0.727273 45.3
rps18-rpl20_p3 AAGAACCGAGTCGACTGCTA TCGATTGTAAAGAATCCAGTGGA 50.0 39.1 58.5 57.6 160-305 bp 0.727273 45.2
rps18-rpl20_p4 TGAAAGAACCGAGTCGACTG GTCGATTGTAAAGAATCCAGTGG 50.0 43.5 57.6 57.5 164-309 bp 0.727273 45.1
rps18-rpl20_p5 AACCGAGTCGACTGCTAGAA GTCGATTGTAAAGAATCCAGTGG 50.0 43.5 58.5 57.5 158-303 bp 0.727273 44.8