Hotspot

psaJ-rpl33

LSC · 67951-68407 · 457 bp

Back to Coffea

Pi 0.0082
MarkerSeek score 42.40
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
457
variable_sites
14
pi
0.0082
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
42.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 ATGCGTTGACATTCCCCTTT TCACCGTTACTCGTACGTCT 45.0 50.0 58.1 58.2 521-526 bp 1.0 80.7
psaJ-rpl33_p2 ATCTCAGTCGATCCCTCGTT TCACCGTTACTCGTACGTCT 50.0 50.0 57.9 58.2 1109-1114 bp 1.0 80.2
psaJ-rpl33_p3 ACCCGTGACATTTTGTACCC TCACCGTTACTCGTACGTCT 50.0 50.0 58.1 58.2 1046-1051 bp 1.0 79.9
psaJ-rpl33_p4 ATCTCAGTCGATCCCTCGTT TTGTGCCGGTTCTTTTGAGT 50.0 45.0 57.9 58.2 1197-1202 bp 1.0 79.7
psaJ-rpl33_p5 TGTACAATTCGAACCGGTCC TCACCGTTACTCGTACGTCT 50.0 50.0 57.9 58.2 714-719 bp 0.727273 69.0