Hotspot

psbE-petL

LSC · 65562-66635 · 1074 bp

Back to Coffea

Pi 0.00891
MarkerSeek score 56.50
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1074
variable_sites
32
pi
0.00891
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 ACGAATAGCCAACCAGCAAT GCTGAGGTTATAGTTAAAGCTGC 45.0 43.5 57.9 57.6 1240-1248 bp 1.0 54.5
psbE-petL_p2 AGAACGTTCTCCTGTGCTTC GCTGAGGTTATAGTTAAAGCTGC 50.0 43.5 57.8 57.6 1154-1162 bp 1.0 54.5
psbE-petL_p3 GTTCTCCTGTGCTTCCAGAC GCTGAGGTTATAGTTAAAGCTGC 55.0 43.5 58.2 57.6 1149-1157 bp 1.0 54.2
psbE-petL_p4 TCTCCTGTGCTTCCAGACAT GCTGAGGTTATAGTTAAAGCTGC 50.0 43.5 58.4 57.6 1147-1155 bp 1.0 53.0
psbE-petL_p5 ACGAATAGCCAACCAGCAAT AGCTGCTAGTAGAAAACCAA 45.0 40.0 57.9 54.3 1223-1231 bp 1.0 52.1