Hotspot

petA-psbJ

LSC · 63908-64783 · 876 bp

Back to Coffea

Pi 0.00955
MarkerSeek score 65.90
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
876
variable_sites
31
pi
0.00955
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 ATCCATTACGTGTCCAAGGC ATTCCGGATTGGGTTCATCC 50.0 50.0 58.0 57.6 1013-1027 bp 1.0 80.2
petA-psbJ_p2 GAGAAGGTTCAATTGGCCGA ATTCCGGATTGGGTTCATCC 50.0 50.0 58.2 57.6 927-941 bp 1.0 79.4
petA-psbJ_p3 TCGAGAAGGTTCAATTGGCC ATTCCGGATTGGGTTCATCC 50.0 50.0 58.2 57.6 929-943 bp 1.0 79.4
petA-psbJ_p4 GGCCTTTTGTTCTTCTTGGC ATTCCGGATTGGGTTCATCC 50.0 50.0 57.8 57.6 996-1010 bp 1.0 79.3
petA-psbJ_p5 CATTACGTGTCCAAGGCCTT ATTCCGGATTGGGTTCATCC 50.0 50.0 58.2 57.6 1010-1024 bp 1.0 79.1