Hotspot

pafII-cemA

LSC · 61153-61998 · 846 bp

Back to Coffea

Pi 0.00642
MarkerSeek score 57.60
Primer available yes
Label pafII-cemA

Feature Metadata

label_name
pafII-cemA
feature_type
igs
region
LSC
length_bp
846
variable_sites
15
pi
0.00642
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
pafII-cemA_p1 TTGGCCTATTTCTTGCGTGT TGAGAGAGAAAGCCACCAGG 45.0 55.0 58.1 59.0 808-963 bp 1.0 76.4
pafII-cemA_p2 TTGGCCTATTTCTTGCGTGT ATGAGAGAGAAAGCCACCAG 45.0 50.0 58.1 56.9 809-964 bp 1.0 76.0
pafII-cemA_p3 TTGGCCTATTTCTTGCGTGT AAGGGGAGTGAATGCTTTCT 45.0 45.0 58.1 56.7 760-915 bp 1.0 74.4
pafII-cemA_p4 TTGGCCTATTTCTTGCGTGT AGGGGAGTGAATGCTTTCTT 45.0 45.0 58.1 56.7 759-914 bp 1.0 74.4
pafII-cemA_p5 TTGGCCTATTTCTTGCGTGT AATGAGAGAGAAAGCCACCAG 45.0 47.6 58.1 57.7 810-965 bp 1.0 73.8