Hotspot

atpB-rbcL

LSC · 54933-55702 · 770 bp

Back to Coffea

Pi 0.00538
MarkerSeek score 58.80
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
770
variable_sites
14
pi
0.00538
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 GGAAACCCCAGAACCAGAAG CACCAGCTTTGAATCCAACAC 55.0 47.6 58.1 58.0 857-883 bp 1.0 73.8
atpB-rbcL_p2 GGAAACCCCAGAACCAGAAG ACCAGCTTTGAATCCAACACT 55.0 42.9 58.1 58.1 856-882 bp 1.0 73.6
atpB-rbcL_p3 GATTTGGACAATACGCCCCA CACCAGCTTTGAATCCAACAC 50.0 47.6 58.2 58.0 896-922 bp 1.0 72.5
atpB-rbcL_p4 TGATTTGGACAATACGCCCC CACCAGCTTTGAATCCAACAC 50.0 47.6 58.2 58.0 897-923 bp 1.0 72.5
atpB-rbcL_p5 GATTTGGACAATACGCCCCA ACCAGCTTTGAATCCAACACT 50.0 42.9 58.2 58.1 895-921 bp 1.0 72.2