Hotspot

trnT-GGU-psbD

LSC · 32193-33354 · 1162 bp

Back to Coffea

Pi 0.00563
MarkerSeek score 60.40
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1162
variable_sites
24
pi
0.00563
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GCCCTTTTAACTCAGCGGTA GAAACGGTCCCTACGTAACC 50.0 55.0 57.9 58.0 1318-1419 bp 1.0 81.4
trnT-GGU-psbD_p2 GCCCTTTTAACTCAGCGGTA GGACCAGCCTACAAAAACGA 50.0 50.0 57.9 58.1 1336-1437 bp 1.0 80.5
trnT-GGU-psbD_p3 GGCGTAAGTCATCGGTTCAA GAAACGGTCCCTACGTAACC 50.0 55.0 58.3 58.0 1281-1382 bp 1.0 80.1
trnT-GGU-psbD_p4 GCCCTTTTAACTCAGCGGTA ACAAAAACGAAACGGTCCCT 50.0 45.0 57.9 58.2 1326-1427 bp 1.0 79.5
trnT-GGU-psbD_p5 GGCGTAAGTCATCGGTTCAA GGACCAGCCTACAAAAACGA 50.0 50.0 58.3 58.1 1299-1400 bp 1.0 79.1