Hotspot

petN-psbM

LSC · 28754-29913 · 1160 bp

Back to Coffea

Pi 0.00672
MarkerSeek score 58.40
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1160
variable_sites
30
pi
0.00672
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 GTCTCGCTTGGGCTTCTTTA TCATTCTAGTTCCTACGGCCT 50.0 47.6 57.9 58.0 1240-1302 bp 1.0 76.8
petN-psbM_p2 TAGTAAGTCTCGCTTGGGCT TCATTCTAGTTCCTACGGCCT 50.0 47.6 58.2 58.0 1246-1308 bp 1.0 76.3
petN-psbM_p3 GTCTCGCTTGGGCTTCTTTA TCTAGTTCCTACGGCCTTTCT 50.0 47.6 57.9 57.9 1236-1298 bp 1.0 76.2
petN-psbM_p4 TAGTAAGTCTCGCTTGGGCT TCTAGTTCCTACGGCCTTTCT 50.0 47.6 58.2 57.9 1242-1304 bp 1.0 75.7
petN-psbM_p5 GTCTCGCTTGGGCTTCTTTA AGTTCCTACGGCCTTTCTACT 50.0 47.6 57.9 58.2 1233-1295 bp 1.0 75.6