Hotspot

trnK-UUU-rps16

LSC · 4130-4997 · 868 bp

Back to Coffea

Pi 0.0103
MarkerSeek score 50.70
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
868
variable_sites
29
pi
0.0103
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT CGCCCTAATCAAATGAAGTTCA 50.0 40.9 58.2 57.0 917-934 bp 1.0 60.2
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT CGCCCTAATCAAATGAAGTTCA 50.0 40.9 58.2 57.0 916-933 bp 1.0 60.2
trnK-UUU-rps16_p3 CCGCACTTAAAAGCCGAGTA CGCCCTAATCAAATGAAGTTCA 50.0 40.9 58.3 57.0 926-943 bp 1.0 59.3
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT GAACTTCGCCCTAATCAAATGA 50.0 40.9 58.2 56.8 922-939 bp 1.0 58.1
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT TCGCCCTAATCAAATGAAGTTC 50.0 40.9 58.2 56.8 917-934 bp 1.0 58.1