Hotspot

trnH-GUG-psbA

LSC · 78-303 · 226 bp

Back to Coffea

Pi 0.0363
MarkerSeek score 69.10
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
226
variable_sites
24
pi
0.0363
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 295-327 bp 1.0 88.7
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG GCGCTAACCTTGGTATGGAA 55.0 50.0 58.1 58.0 342-374 bp 1.0 88.2
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 356-388 bp 1.0 87.9
trnH-GUG-psbA_p4 AATCCACTGCCTTGATCCAC GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 355-387 bp 1.0 87.9
trnH-GUG-psbA_p5 ACAATCCACTGCCTTGATCC GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 357-389 bp 1.0 87.9