Hotspot

ndhA

SSC · 124775-126982 · 2208 bp

Back to Coelogyne

Pi 0.0123

MarkerSeek score 80.40

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2208
variable_sites: 245
pi: 0.0123
unique_haplotype_count: 52
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGTTTCTTTCTCTTGGTTGAGA	TTGCAAATTCTTCCCCAGCT	36.4	45.0	55.5	58.0	80-2544 bp	0.942308	53.4
ndhA_p2	TGTTTCTTTCTCTTGGTTGAGA	GTCCACCCGGTTTCATCAAT	36.4	50.0	55.5	58.2	80-2564 bp	0.942308	52.2
ndhA_p3	ATGTTTCTTTCTCTTGGTTGAGA	TTGCAAATTCTTCCCCAGCT	34.8	45.0	56.0	58.0	80-2545 bp	0.942308	49.6
ndhA_p4	TGTTTCTTTCTCTTGGTTGAGAT	TTGCAAATTCTTCCCCAGCT	34.8	45.0	56.0	58.0	80-2544 bp	0.942308	49.6
ndhA_p5	TGTTTCTTTCTCTTGGTTGAG	TTGCAAATTCTTCCCCAGCT	38.1	45.0	54.0	58.0	80-2544 bp	0.942308	49.4