Hotspot

rpl32-trnL-UAG

SSC · 118115-118687 · 573 bp

Back to Coelogyne

Pi 0.0293

MarkerSeek score 76.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 573
variable_sites: 127
pi: 0.0293
unique_haplotype_count: 52
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TCCACCGGACAGTCAAAAAG	ATCCGCATAAAAGAGCTGCA	50.0	45.0	58.0	58.0	1231-2277 bp	1.0	91.4
rpl32-trnL-UAG_p2	TCCACCGGACAGTCAAAAAG	TCCGCATAAAAGAGCTGCAT	50.0	45.0	58.0	58.0	1230-2278 bp	1.0	91.4
rpl32-trnL-UAG_p3	TCCACCGGACAGTCAAAAAG	TGGCGAAACCTTGGGTAAAA	50.0	45.0	58.0	57.9	1111-2275 bp	1.0	91.1
rpl32-trnL-UAG_p4	TCCACCGGACAGTCAAAAAG	GTCCCGAAGAAATCCAACGA	50.0	50.0	58.0	57.9	964-2272 bp	1.0	91.0
rpl32-trnL-UAG_p5	TCCACCGGACAGTCAAAAAG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	591-2277 bp	1.0	90.7