Hotspot

rpl20-rps12

LSC · 71738-72473 · 736 bp

Back to Coelogyne

Pi 0.0095

MarkerSeek score 73.00

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 736
variable_sites: 78
pi: 0.0095
unique_haplotype_count: 43
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCCGAGCTGTATATCCTCGT	ACTAGGGTGTATGTGCGACT	50.0	50.0	57.4	58.2	754-815 bp	1.0	86.0
rpl20-rps12_p2	TCCGAGCTGTATATCCTCGT	GAAATCTCCCGCTCTTCGAG	50.0	55.0	57.4	58.2	800-861 bp	1.0	85.6
rpl20-rps12_p3	TCCGAGCTGTATATCCTCGT	TAGAAACGCAAGACAGCCAA	50.0	45.0	57.4	57.8	833-894 bp	1.0	84.8
rpl20-rps12_p4	TCCGAGCTGTATATCCTCGT	TTAGAAACGCAAGACAGCCA	50.0	45.0	57.4	57.8	834-895 bp	1.0	84.7
rpl20-rps12_p5	CGACGTCTCCGAGCTGTATA	ACTAGGGTGTATGTGCGACT	55.0	50.0	58.8	58.2	761-822 bp	1.0	84.2