Hotspot

petA-psbJ

LSC · 65458-66553 · 1096 bp

Back to Coelogyne

Pi 0.0213

MarkerSeek score 73.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1096
variable_sites: 175
pi: 0.0213
unique_haplotype_count: 49
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGAAAAGGTTCAGTTGTACGA	TTCCTATTCCGGGTTGGGTT	38.1	50.0	55.4	58.6	591-1167 bp	1.0	58.3
petA-psbJ_p2	TGAAAAGGTTCAGTTGTACGA	TATTCCGGGTTGGGTTCATC	38.1	50.0	55.4	57.3	587-1163 bp	1.0	57.5
petA-psbJ_p3	TGAAAAGGTTCAGTTGTACGA	CTATTCCGGGTTGGGTTCAT	38.1	50.0	55.4	57.3	588-1164 bp	1.0	57.5
petA-psbJ_p4	ACAGTTTGAAAAGGTTCAGTTGT	TTCCTATTCCGGGTTGGGTT	34.8	50.0	57.3	58.6	597-1173 bp	1.0	57.2
petA-psbJ_p5	AGGTTCAGTTGTACGAAATGA	TTCCTATTCCGGGTTGGGTT	38.1	50.0	55.2	58.6	586-1162 bp	0.980769	55.7