Hotspot

atpB-rbcL

LSC · 56077-57145 · 1069 bp

Back to Coelogyne

Pi 0.0332

MarkerSeek score 81.90

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 1069
variable_sites: 250
pi: 0.0332
unique_haplotype_count: 51
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ACGCCCTAGGTTTTGGTTTT	GGTGACATAAGTCCCTCCCT	45.0	55.0	57.9	58.1	821-1228 bp	1.0	90.4
atpB-rbcL_p2	TGGGCCAATAATTTGAGCGA	GGTGACATAAGTCCCTCCCT	45.0	55.0	57.9	58.1	842-1249 bp	0.961538	88.4
atpB-rbcL_p3	ACGCCCTAGGTTTTGGTTTT	TGGTGACATAAGTCCCTCCC	45.0	55.0	57.9	58.4	822-1229 bp	1.0	88.0
atpB-rbcL_p4	CGATACGCCCTAGGTTTTGG	GGTGACATAAGTCCCTCCCT	55.0	55.0	58.4	58.1	833-1232 bp	0.980769	86.9
atpB-rbcL_p5	CGCCCTAGGTTTTGGTTTTG	GGTGACATAAGTCCCTCCCT	50.0	55.0	57.6	58.1	820-1227 bp	0.980769	86.8