Hotspot

rpoB-trnC-GCA

LSC · 27189-28685 · 1497 bp

Back to Coelogyne

Pi 0.0197

MarkerSeek score 72.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1497
variable_sites: 237
pi: 0.0197
unique_haplotype_count: 51
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AATTCTTCCGTCAAGCCCTG	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.2	57.5	1494-1680 bp	1.0	86.4
rpoB-trnC-GCA_p2	AAATTCTTCCGTCAAGCCCT	TAAAGGATTTGCAGTCCCCC	45.0	50.0	57.4	57.5	1495-1681 bp	1.0	83.1
rpoB-trnC-GCA_p3	AATTCTTCCGTCAAGCCCTG	ATAAAGGATTTGCAGTCCCCC	50.0	47.6	58.2	58.0	1495-1681 bp	1.0	82.4
rpoB-trnC-GCA_p4	CTTCCGTCAAGCCCTGATTA	TAAAGGATTTGCAGTCCCCC	50.0	50.0	57.3	57.5	1490-1676 bp	1.0	82.3
rpoB-trnC-GCA_p5	GAAATTCTTCCGTCAAGCCC	TAAAGGATTTGCAGTCCCCC	50.0	50.0	57.1	57.5	1496-1682 bp	1.0	80.4