Hotspot

rrn23

Genome · 176374-178446 · 2073 bp

Back to Coelastrum

Pi 0.0652
MarkerSeek score 83.00
Primer available yes
Label rrn23

Feature Metadata

label_name
rrn23
feature_type
rRNA
region
Genome
length_bp
2073
variable_sites
240
pi
0.0652
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn23_p1 AGATTGTAGGCTGCAACTCG CTGTACGAGGAGTTTCCAGC 50.0 55.0 58.0 58.0 2870 bp 0.2 47.9
rrn23_p2 AGATTGTAGGCTGCAACTCG GCTGTACGAGGAGTTTCCAG 50.0 55.0 58.0 58.0 2871 bp 0.2 47.9
rrn23_p3 AGATTGTAGGCTGCAACTCG TGATTGTCCCCCTGTCTTCT 50.0 50.0 58.0 58.0 2986 bp 0.2 47.5
rrn23_p4 CGAGAGGTCACTGGTTCAAG CTGTACGAGGAGTTTCCAGC 55.0 55.0 57.9 58.0 2456 bp 0.2 47.4
rrn23_p5 CGAGAGGTCACTGGTTCAAG GCTGTACGAGGAGTTTCCAG 55.0 55.0 57.9 58.0 2457 bp 0.2 47.4
rrn23_p1 CTGTACGAGGAGTTTCCAGC AGATTGTAGGCTGCAACTCG 55.0 50.0 58.0 58.0 2870 bp 0.2 47.9
rrn23_p2 GCTGTACGAGGAGTTTCCAG AGATTGTAGGCTGCAACTCG 55.0 50.0 58.0 58.0 2871 bp 0.2 47.9
rrn23_p3 TGATTGTCCCCCTGTCTTCT AGATTGTAGGCTGCAACTCG 50.0 50.0 58.0 58.0 2986 bp 0.2 47.5
rrn23_p4 CTGTACGAGGAGTTTCCAGC CGAGAGGTCACTGGTTCAAG 55.0 55.0 58.0 57.9 2456 bp 0.2 47.4
rrn23_p5 GCTGTACGAGGAGTTTCCAG CGAGAGGTCACTGGTTCAAG 55.0 55.0 58.0 57.9 2457 bp 0.2 47.4