Hotspot

rpoA

Genome · 142772-144244 · 1473 bp

Back to Coelastrum

Pi 0.239

MarkerSeek score 83.50

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: Genome
length_bp: 1473
variable_sites: 628
pi: 0.239
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	GCAACTGAACTATACCCGCA	CTTAGCTCAGTTGGCCAGAG	50.0	55.0	58.0	58.0	2415-2760 bp	0.8	83.7
rpoA_p2	GCAACTGAACTATACCCGCA	GCCAGAGCATCCGTTTCATA	50.0	50.0	58.0	58.0	2402-2747 bp	0.8	83.5
rpoA_p3	GCAACTGAACTATACCCGCA	ACCAGTTCGCCTTCACAAAT	50.0	45.0	58.0	58.0	2176 bp	0.2	47.7
rpoA_p4	GCAACTGAACTATACCCGCA	ATTCGAAAAGTGCCACACCT	50.0	45.0	58.0	58.0	2239 bp	0.2	47.7
rpoA_p5	GCAACTGAACTATACCCGCA	CGATTCGAAAAGTGCCACAC	50.0	50.0	58.0	58.0	2241 bp	0.2	47.5