Hotspot

rbcL

Genome · 63027-64454 · 1428 bp

Back to Coelastrum

Pi 0.0981

MarkerSeek score 83.90

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: Genome
length_bp: 1428
variable_sites: 296
pi: 0.0981
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	GATTTACAATCCGCCCCCAT	GATCGGCGGTTAAAAGAGGT	50.0	50.0	58.0	58.0	2008 bp	0.2	47.8
rbcL_p2	GATTTACAATCCGCCCCCAT	TCCACCTGCAGAAAGAGGTA	50.0	50.0	58.0	58.0	1977 bp	0.2	47.8
rbcL_p3	TCGCTGCTTCTTTCTTCGAA	GATCGGCGGTTAAAAGAGGT	45.0	50.0	57.9	58.0	2101 bp	0.2	46.6
rbcL_p4	TTCGCTGCTTCTTTCTTCGA	GATCGGCGGTTAAAAGAGGT	45.0	50.0	57.9	58.0	2102 bp	0.2	46.6
rbcL_p5	TCGCTGCTTCTTTCTTCGAA	TCCACCTGCAGAAAGAGGTA	45.0	50.0	57.9	58.0	2070 bp	0.2	46.6