Hotspot

psbC

Genome · 85980-87365 · 1386 bp

Back to Coelastrum

Pi 0.474

MarkerSeek score 70.80

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: Genome
length_bp: 1386
variable_sites: 783
pi: 0.474
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.37
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	AAATGTCTGCGCTTTTCTGC	TTTCGGCATTGAGCTCTCTC	45.0	50.0	57.9	58.0	1851 bp	0.2	47.4
psbC_p2	AAATGTCTGCGCTTTTCTGC	TCTCGTACGACTTGCATGTG	45.0	50.0	57.9	58.0	2282 bp	0.2	47.4
psbC_p3	AAATGTCTGCGCTTTTCTGC	GTGTTTTCGGCATTGAGCTC	45.0	50.0	57.9	58.0	1855 bp	0.2	47.3
psbC_p4	AAATGTCTGCGCTTTTCTGC	AGTTCTTACGCGTTCCTCAC	45.0	50.0	57.9	57.9	2344 bp	0.2	47.1
psbC_p5	AAATGTCTGCGCTTTTCTGC	GCTGTTATCCCCCACCAAAA	45.0	50.0	57.9	58.1	2368 bp	0.2	47.0