Hotspot

clpP

Genome · 116611-118257 · 1647 bp

Back to Coelastrum

Pi 0.114

MarkerSeek score 81.60

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: Genome
length_bp: 1647
variable_sites: 357
pi: 0.114
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	ATTGCTTCTCTGCTTCAGGG	GCAAAACGCGTATTCTAGGTG	50.0	47.6	57.9	58.0	2023 bp	0.2	38.7
clpP_p2	ATTGCTTCTCTGCTTCAGGG	CAATTTGACAGGTTTTCCGCA	50.0	42.9	57.9	57.9	2222 bp	0.2	38.3
clpP_p3	AGCCTTCGTGATGAAACTGG	GCAAAACGCGTATTCTAGGTG	50.0	47.6	58.2	58.0	1932 bp	0.2	38.2
clpP_p4	ATTGCTTCTCTGCTTCAGGG	ACGCGTATTCTAGGTGTTTCC	50.0	47.6	57.9	58.1	2018 bp	0.2	38.1
clpP_p5	ATTGCTTCTCTGCTTCAGGG	CGCGTATTCTAGGTGTTTCCA	50.0	47.6	57.9	58.1	2017 bp	0.2	38.1