Hotspot

chlN

Genome · 23515-25053 · 1539 bp

Back to Coelastrum

Pi 0.158

MarkerSeek score 82.50

Primer available yes

Label chlN

Feature Metadata

label_name: chlN
feature_type: gene
region: Genome
length_bp: 1539
variable_sites: 443
pi: 0.158
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlN_p1	GAACAAAAGACAAGCAGGACA	TGCAAATTTCGACTCGAGTT	42.9	40.0	56.9	56.0	2300 bp	0.2	15.6
chlN_p2	TGAACAAAAGACAAGCAGGAC	TGCAAATTTCGACTCGAGTT	42.9	40.0	56.9	56.0	2301 bp	0.2	15.6
chlN_p3	GAACAAAAGACAAGCAGGACA	ATGCAAATTTCGACTCGAGT	42.9	40.0	56.9	55.8	2301 bp	0.2	13.8
chlN_p4	TGAACAAAAGACAAGCAGGAC	ATGCAAATTTCGACTCGAGT	42.9	40.0	56.9	55.8	2302 bp	0.2	13.8
chlN_p5	ACAAAAGACAAGCAGGACAA	TGCAAATTTCGACTCGAGTT	40.0	40.0	55.7	56.0	2298 bp	0.2	13.7