Hotspot

ccsA

Genome · 135347-136459 · 1113 bp

Back to Coelastrum

Pi 0.188

MarkerSeek score 83.60

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: Genome
length_bp: 1113
variable_sites: 371
pi: 0.188
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GGCGAACAAAAATGCATCTCA	TCAGGTATTTCTGCGTTGCA	42.9	45.0	58.1	57.8	1625 bp	0.2	38.1
ccsA_p2	ACAAAGGCGAACAAAAATGCA	TCAGGTATTTCTGCGTTGCA	38.1	45.0	58.1	57.8	1630 bp	0.2	37.8
ccsA_p3	AGGCGAACAAAAATGCATCTC	TCAGGTATTTCTGCGTTGCA	42.9	45.0	57.8	57.8	1626 bp	0.2	36.8
ccsA_p4	AGGCGAACAAAAATGCATCT	TCAGGTATTTCTGCGTTGCA	40.0	45.0	56.6	57.8	1626 bp	0.2	35.4
ccsA_p5	AAGGCGAACAAAAATGCATCT	TCAGGTATTTCTGCGTTGCA	38.1	45.0	57.3	57.8	1627 bp	0.2	33.3