Hotspot

ycf1-trnS-GCU

Genome · 169021-172773 · 3753 bp

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Pi 0.39

MarkerSeek score 67.30

Primer available yes

Label ycf1-trnS-GCU

Feature Metadata

label_name: ycf1-trnS-GCU
feature_type: igs
region: Genome
length_bp: 3753
variable_sites: 1874
pi: 0.39
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.40
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnS-GCU_p1	TTTTCAATCAGCCTGCTTCG	TCGCAATACGTGAAATCCGT	45.0	45.0	57.0	58.0	667 bp	0.2	40.0
ycf1-trnS-GCU_p2	TTTTCAATCAGCCTGCTTCG	TCGTGTGATTCGCAATACGT	45.0	45.0	57.0	58.0	676 bp	0.2	39.9
ycf1-trnS-GCU_p3	TTTTCAATCAGCCTGCTTCG	GCCAAAAGACGTCAAAGTCG	45.0	50.0	57.0	58.0	607 bp	0.2	39.9
ycf1-trnS-GCU_p4	TTTTCAATCAGCCTGCTTCG	ATCGCATGGACTGAGAAACC	45.0	50.0	57.0	58.0	343 bp	0.2	39.8
ycf1-trnS-GCU_p5	TTTTCAATCAGCCTGCTTCG	TTCGAAAGACAACGAAGCCA	45.0	45.0	57.0	58.1	755 bp	0.2	39.6