Hotspot

rpl12-cemA

Genome · 99829-104483 · 4655 bp

Back to Coelastrum

Pi 0.37

MarkerSeek score 62.50

Primer available yes

Label rpl12-cemA

Feature Metadata

label_name: rpl12-cemA
feature_type: igs
region: Genome
length_bp: 4655
variable_sites: 537
pi: 0.37
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.12
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl12-cemA_p1	CCTACCCCTGAGGAAAAAGC	GGAGTCTTTCAGTGTCAGGC	55.0	55.0	57.9	58.2	568 bp	0.2	45.4
rpl12-cemA_p2	CCTACCCCTGAGGAAAAAGC	GCACAAACTGCACACATTGA	55.0	45.0	57.9	57.8	1073 bp	0.2	45.3
rpl12-cemA_p3	GAGAGCTTCAAACCTGCTGA	GGAGTCTTTCAGTGTCAGGC	50.0	55.0	57.8	58.2	939 bp	0.2	45.0
rpl12-cemA_p4	AGAGAGCTTCAAACCTGCTG	GGAGTCTTTCAGTGTCAGGC	50.0	55.0	57.8	58.2	940 bp	0.2	45.0
rpl12-cemA_p5	GAGAGCTTCAAACCTGCTGA	GCACAAACTGCACACATTGA	50.0	45.0	57.8	57.8	1444 bp	0.2	44.9