Hotspot

rrn23-rrn23

Genome · 93935-95372 · 1438 bp

Back to Coelastrum

Pi 0.161

MarkerSeek score 82.10

Primer available yes

Label rrn23-rrn23

Feature Metadata

label_name: rrn23-rrn23
feature_type: igs
region: Genome
length_bp: 1438
variable_sites: 368
pi: 0.161
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn23-rrn23_p1	GAAGGGCCGTCGCTCAAC	TTCACTACAGGTCCTCTCGT	66.7	50.0	61.1	57.4	1080-1561 bp	0.8	44.0
rrn23-rrn23_p2	GAAGGGCCGTCGCTCAAC	ATGGACCGAACTGTCTTACG	66.7	50.0	61.1	57.4	1017-1498 bp	0.8	44.0
rrn23-rrn23_p3	GAAGGGCCGTCGCTCAAC	GTTCACTACAGGTCCTCTCG	66.7	55.0	61.1	57.1	1081-1562 bp	0.8	44.0
rrn23-rrn23_p4	GAAGGGCCGTCGCTCAAC	CACTACAGGTCCTCTCGTACT	66.7	52.4	61.1	58.0	1078-1559 bp	0.8	44.0
rrn23-rrn23_p5	GAAGGGCCGTCGCTCAAC	TCACTACAGGTCCTCTCGTAC	66.7	52.4	61.1	58.0	1079-1560 bp	0.8	44.0