Hotspot

rpl20-psbA

Genome · 65940-67122 · 1183 bp

Back to Coelastrum

Pi 0.458

MarkerSeek score 74.80

Primer available yes

Label rpl20-psbA

Feature Metadata

label_name: rpl20-psbA
feature_type: igs
region: Genome
length_bp: 1183
variable_sites: 707
pi: 0.458
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.68
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-psbA_p1	ACGAGCAATCCATACACGTC	GAAACGTTGGTTCTCATGCG	50.0	50.0	58.1	58.0	2122 bp	0.2	47.0
rpl20-psbA_p2	ACGAGCAATCCATACACGTC	GCTGCTTTTTCAACGTGGTT	50.0	45.0	58.1	58.1	1982 bp	0.2	46.8
rpl20-psbA_p3	ACGAGCAATCCATACACGTC	GGTTGCTGCTTTTTCAACGT	50.0	45.0	58.1	58.1	1986 bp	0.2	46.8
rpl20-psbA_p4	ACGAGCAATCCATACACGTC	TGGTTGCTGCTTTTTCAACG	50.0	45.0	58.1	58.1	1987 bp	0.2	46.7
rpl20-psbA_p5	ACGAGCAATCCATACACGTC	TTGCTGCTTTTTCAACGTGG	50.0	45.0	58.1	58.1	1984 bp	0.2	46.7