Hotspot

infA-psbB

Genome · 55830-57246 · 1417 bp

Back to Coelastrum

Pi 0.449

MarkerSeek score 74.00

Primer available yes

Label infA-psbB

Feature Metadata

label_name: infA-psbB
feature_type: igs
region: Genome
length_bp: 1417
variable_sites: 666
pi: 0.449
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.60
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-psbB_p1	GGGGTTGAAGTTATTGGGCA	AAAGTGTCATCGATCCAGCC	50.0	50.0	58.1	58.0	1670 bp	0.2	47.2
infA-psbB_p2	GGGGTTGAAGTTATTGGGCA	CACCGCTAATTGTCCATCCA	50.0	50.0	58.1	58.0	1799 bp	0.2	47.2
infA-psbB_p3	GGGGTTGAAGTTATTGGGCA	TTTGTAGCTGTTTCACCGCT	50.0	45.0	58.1	58.0	1812 bp	0.2	47.1
infA-psbB_p4	GGGGTTGAAGTTATTGGGCA	ACCGCTAATTGTCCATCCAC	50.0	50.0	58.1	58.0	1798 bp	0.2	47.1
infA-psbB_p5	GGGGTTGAAGTTATTGGGCA	TGTTACGTGGAAAGCACCAA	50.0	45.0	58.1	58.0	2029 bp	0.2	47.1