Hotspot

psbD-trnS-UGA

Genome · 39586-40684 · 1099 bp

Back to Coelastrum

Pi 0.401

MarkerSeek score 73.80

Primer available yes

Label psbD-trnS-UGA

Feature Metadata

label_name: psbD-trnS-UGA
feature_type: igs
region: Genome
length_bp: 1099
variable_sites: 338
pi: 0.401
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.40
markerseek_score: 73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnS-UGA_p1	GCATCATCAAACCATGTACGT	GGTTCGAATCCCTCCCTTTC	42.9	55.0	57.0	58.0	361-1177 bp	1.0	75.4
psbD-trnS-UGA_p2	GCATCATCAAACCATGTACGT	GTTCGAATCCCTCCCTTTCC	42.9	55.0	57.0	58.0	360-1176 bp	1.0	75.4
psbD-trnS-UGA_p3	GCATCATCAAACCATGTACGTT	GGTTCGAATCCCTCCCTTTC	40.9	55.0	57.6	58.0	361-1177 bp	1.0	72.8
psbD-trnS-UGA_p4	GCATCATCAAACCATGTACGTT	GTTCGAATCCCTCCCTTTCC	40.9	55.0	57.6	58.0	360-1176 bp	1.0	72.8
psbD-trnS-UGA_p5	GCATCATCAAACCATGTACG	GTTCGAATCCCTCCCTTTCC	45.0	55.0	55.2	58.0	360-1176 bp	1.0	68.9